Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDRELVLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPGFELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKD-NIWRVTSRGTSDQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLR-TDSIPIGVKENAAGSAFDFQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDASSVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVTEFKIKTRKE 1NSU Chain:B ((2-339)) -SIKIRDFGLGSDLISLTNKAGVTISFTNLGARIVDWQKDGKHLILGFDSAKEYLEKDAYPGATVGPTAGRIKDGLVKISGKDYILNQNE-GPQTLNGGEESIHTKLWTYEVTDLGAEVQVKFSLVSNDGTNGYPGKIEMSVTHSF-DDDNKWKIHYEAISDKDTVFNPTGHVYFNLNGDASESVENHGLRLAASRFVPLKDQTEIVRGDIVDIKNTDLDFRQEKQLSNAFNSNMEQVQLVKGIDHPFLLDQLGLDKEQARLTLD--DTSISVFTDQPSIVIFTANFGDLGTLYHEKKQVHHGGITFECQVSPGSEQIPELGDISLKAGEKYQATTIYSLHTK--

General information: TITO was launched using: RESULT: Template: 1NSU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2124 -11097 -5.22 -33.03 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -5.22 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_1NSU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NSU-query.scw PDB file : Tito_Scwrl_1NSU.pdb: