Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKISTYENKKVLVLGLAKSG-VSAAKLLHELGALVTVNDGKPFDENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITEVELAYEVA-ECPIIGITGTNGKTTTTTMTGLLLNAGADQGI-ARLAGNIGYPASGVAQEAKSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHG-TRKEYVKAKWNLQKNMTEKDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVY---AD----DYSIYYKKEKIMEISELGVPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVG----EIQGRKFYNDSKATNILATKMALSGFET----SKVVLLAGGLD----RGNTFDELIPSLKGIKAMVVF-----------GQTKEKLMDAGKKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVNRLK 1P3D Chain:A ((14-466)) ----EMRRVQQIHFIGIGGAGMSGIAEILLNEGYQISGSDIADGVV-TQRLA--QAGAKIYIG-HAEEHI-EGASVVVVSSAIKDDNPELVTSKQKRIPVIQRAQMLAEIMRFRHGIAVAGTHGKTTTTAMISMIYT-QA-KLDPTFVNGGLVKSAGKNA-HLGASRYLIAEADESDASFL-HLQPMVSVVTNMEPDHMDTYEGDFEKMKATYVKFLHNLPFYGLAVMCADDPVLMELVPKVGRQVITYGFSEQADYRIEDYEQTGFQGHYTVICP-NNERINVLLNVPGKHNALNATAALAVAKEEGIANEAILEALADFQGAGRRFDQLGEFIRPNGKVRLVDDY-GHHPTEVGVTIKAAREGWGDKRIVMIFQPHRYSRTRDL-FDDFVQVLSQVDALIMLDVYAAGEAPIVGADSKSLCRSIRNLGKVDPILVSDTSQLGDVLDQIIQDGDLILAQGAGSVSKIS------------------

General information: TITO was launched using: RESULT: Template: 1P3D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2511 23277 9.27 55.55 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 9.27 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_1P3D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1P3D-query.scw PDB file : Tito_Scwrl_1P3D.pdb: