Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQASPVYQGSCLCQGIRYEIKGDIGDIIQCHCQRCRKSNGTAFATNAPIN-----SADFKITQGEDLIKKFAVNGV-------------YRWFCSECGSPLISSRDAQPELYRL-------------RIGTLDTPL-DQKPTMHIFAASKAEWECIHDDLPQYDERP 3E9U Chain:A ((548-709)) --MGRGSEFGIFAFTDSVFEITESVGRF-ELKVMRYSGARGTVIVPYWTENDTATESKDYEGARGELVFENNESEKFIDLFILEESSYEKDVSFKVHIGEPRLAPDDGLAAKIKEVEKKPVQDLTELDRILLLSKPRNGELTTAYVRIRESQEFKATVDKLVAKA---

General information: TITO was launched using: RESULT: Template: 3E9U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 450 3750 8.33 28.85 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 8.33 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.144

(partial model without unconserved sides chains): PDB file : Tito_3E9U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E9U-query.scw PDB file : Tito_Scwrl_3E9U.pdb: