Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTSTAKHNYAPDILGAGYEQLTLNFPDDYEGKVVATLVRKKATQPTQKAVLYIHGF---LDYFFQTEMAEQFNAHGYDFYALDLRKYGRSKLPHQIFYNVLDLIEYDAEITQALEIIGQEK-HTQVLLAGHSTGGLTATLYAAHHPDHPLIKALWANSPFYDFNLSLVEKKFGIPMLSRVGKYLPKVKFPSQLNKWYTTSLHKQLKGEWDFNLDWKPTSAPTVQLSFVHAIHTAQKEIHRGVKLNIPALIMHSHQTKNPRKWGPDATQSDVILDVKDIAKFGKKMKGDVSVVSIHNGLHDLVLSAQPVREQVYQQLFQWLDQKVT 2WTM Chain:A ((2-251)) ----------------------GAMYIDCDGIKLNAYLDMPKNNPEKCPLCIIIHGFTGHSEERHIVAVQETLNEIGVATLRADMYGHGKSDGK----FEDHTLFKWLTNILAVVDYAKKLDFVTDIYMAGHSQGGLSVMLAAAMERDI--IKALIPLSPAAMIPEIARTGELLGLKFDPENIPDELDAWDG-----------------------------RKLKGNYVRVAQT-IRVEDFVDKYTKPVLIVHG--------------DQDEAVPYEASVAFSKQYK-NCKLVTIPGDTHCYDHHLELVTEAVKEFMLEQIAK---

General information: TITO was launched using: RESULT: Template: 2WTM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1466 -42752 -29.16 -173.79 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -29.16 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.416

(partial model without unconserved sides chains): PDB file : Tito_2WTM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WTM-query.scw PDB file : Tito_Scwrl_2WTM.pdb: