Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNATLNYQPHWVREDFVDFVLQKINATWAWKRVLAEVTAVQSLHTDMVLIKLKPNRNFNFDQVRAGESILLTLLIDGVYQQRSYSIIEVT-TQGEIALGIKVQ--GLVSRAAQLLHVGECVEISQPQGDFTLHQG-QQPAILIASGSGVTAIYSLLQQALKQQL--EQIHVIYFNRA---EIFHAELKALAEQYPQLQYHFFNTTEQ------KQHLTESLLQKLVP-DFEQTATYVCGHHGMMQQANEIYTQKGAQ-SQLHQEYFQPLQVTGTHAAQPVIFRRAQQEFLAETNLLGSAEQAGLRPQHGCRMGVCNKCSCTKVSGVTQNLLTGEIEDQPNRPIKLCVSQALSPVTIDL 2EIX Chain:A ((9-242)) -----------------------------NEYKKFMLREKQIINHNTRLFRFNLHHPEDVVGLPIGQHMSVKATVDGKEIYRPYTPVSSDDEKGYFDLIIKVYEKGQMSQYIDHLNPGDFLQVRGPKGQFDYKPNMVKEMGMIAGGTGITPMLQVARAIIKNPKEKTIINLIFANVNEDDILLRTELDDMAKKYSNFKVYYVLNNPPAGWTGGVGFVSADMIKQHFSPPSSDIKVMMCGPPMMNKAMQGHLETLGYTPEQWFI-----------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2EIX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1049 -17129 -16.33 -78.94 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -16.33 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_2EIX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EIX-query.scw PDB file : Tito_Scwrl_2EIX.pdb: