Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLIFLPGASGSTTFWHPLIEKLPQQYRAKIIGYPSFGDTPESLEVKSFEDLTNYVVN---QI-HDESVIIAQSMGGIFAVTAALQKPQLVKGLVLIATSGGINLEP----FNV--------QD-----------WRE-AYRQ-----AF----LKYP-----------DWFIT--------------TNANYEEFLSDINIKTLLIWGDNDPVSPVQVGQYLNQKFENSTLYVVKGGDHQLAEKYADEVAVQIKNYLKGLM 3G9X Chain:A ((33-293)) TPVLFLHGNPTSSYLWRNIIPHVAPSHRCIAPDLIGMGKSDKPDLDYFFDDHVRYLDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIACMEFIRPFPTWDEWPEFARETFQAFRTADVGRELIIDQNAFIEGALPKCVVRPLTEVEMDHYREPFLKPVDREPLWRFPNELPIAGEPANIVALVEAYMNWLHQSPVPKLLFWGTPGVLIPPAEAARLAESLPNCKTVDIGPGLHYLQEDNPDLIGSEIARWLPAL-

General information: TITO was launched using: RESULT: Template: 3G9X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 900 -42750 -47.50 -214.82 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -47.50 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_3G9X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G9X-query.scw PDB file : Tito_Scwrl_3G9X.pdb: