Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLRKMKIPFLLLFFLWEAESHAASRPNIILVMADDLGIGDPGCYGNKTIRTPNIDRLASGGVKLTQHLAASPLCTPSRAAFMTGRYPVRSGMASWSRTGVFLFTASSGGLPTDEITFAKLLKDQGYSTALIGKWHLGMSCHSKTDFCHHPLHHGFNYFYGISLTNLRDCKPGEGSVFTTGFKRLVFLPLQIVGVTLLTLAALNCLGLLHVPLGVFFSLLFLAALILTLFLGFLHYFRPLNCFMMRNYEIIQQPMSYDNLTQRLTVEAAQFIQRNTET--PFLLVLSYLHVHTALFSSKDFAGKSQHGVYGDAVEEMDWSVGQILNLLDELRLANDTLIYFTSDQGAHVEEVSSKGEIHGGSNGIYKGGKANNWEGGIRVPGILRWPRVIQAGQKIDEPTSNMDIFPTVAKLAGAPLPEDRIIDGRDLMPLLEGKSQRSDHEFLFHYCNAYLN----AVRWHPQNSTSIWKAFFFTPNFNPVGSNGCFATHVCFCFGSYVTHHDPPLLFDISKDPRERNPLTPASEPRFYEILKVMQEAADRHTQTLPEVPDQFSWNNFLWKPWLQLC----CPSTGLSCQCDREKQDKRLSR 1E3C Chain:P ((1-485)) ------------------------RPPNIVLIFADDLGYGDLGCYGHPSSTTPNLDQLAAGGLRFTDFYVPVSLSTPSRAALLTGRLPVRMGMYPGV-----LVPSSRGGLPLEEVTVAEVLAARGYLTGMAGKWHLGVGPEGA----FLPPHQGFHRFLGIPYSH-DQGPCQNLTCFPPA---------------------------------------------TPCDGGCDQGLVPIP--LLANLSVEAQPPWLPGLEARYMAFAHDLMADAQREDRPFFLYYASHHTHYPQFSGQSFAERSGRGPFGDSLMELDAAVGTLMTAIGDLGLLEETLVIFTADNGPETMR-----MSRGGCSGLLRCGKGTTYEGGVREPALAFWPGHIAPGVT-HELASSLDLLPTLAALAGAPLPNVTL-DGFDLSPLLLGTGKSPRQSLFFYPSYPDEVRGVFAVRTGK------YKAHFFTQGSAHSDTTADPACHA----SSSLTAHEPPLLYDLSKDPGENYNL-LGATPEVLQALKQLQLLKAQLDAAVTFGPSQVARGE---DPALQICCHPGCTPRPACCHCP----------

General information: TITO was launched using: RESULT: Template: 1E3C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 3137 -45675 -14.56 -96.97 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain P : 0.72 3D Compatibility (PKB) : -14.56 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_1E3C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E3C-query.scw PDB file : Tito_Scwrl_1E3C.pdb: