Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLHGKRKEIYKYEAPWTVYAMNWSVRPDKRFRLALGSFVEEYNNKVQLVGLDEESSEFICRNTF-----DHPYPTTKLMWIPDTKGVYPDLLATSGD-YLRVWRV-GETETRLECLLNNNKNSDFCAPLTSFDWNEVDPYLLGTSSIDTTCTIWGLETGQVLGRV------------------------------NLVSGHVKTQLIAHDKEVYDIAFSRAGGGRDMFASVGADGSVRMFDLRHLEHSTIIYEDPQHHPLLRLCWNKQDPNYLATMAMDGMEVVILDVRVPCTPVARLNNHRACVNGIAWAPHSSCHICTAADDHQALIWDIQQMPRAIE-DPILAYTAEGEINNVQWASTQPDWIAICYNNCLEILRV----- 1R5M Chain:A ((34-425)) ----GFVKILKEIVKLDNIVSSTWN--PLDESILAYGE----KNSVARLARIVETYWKLTIIAELRHPFALSTNQVTCLAWSHDGN----SIVTGVENGELRLWNKTGALLNVLN---------FHRAPIVSVKWNK-DGTHIISMDVENVTILWNVISGTVMQHFELKGSLGVDVEWVDDDKFVIPGPKGAIFVYQITEKTPTGKLIGHHGPISVLEFNDTNK---LLLSASDDGTLRIWHGGNGNSQNCFYGHSQSIVSASWVGD----DKVISCSMDG--SVRLWSLKQNTLLALSIVDGVPIFAGRISQDGQ-KYAVAFMDGQVNVYDLKKLNSPLPIPLYASYQSSQDNDYIFDLSWNCAGNKISVAYSLQEGSVVAIPG

General information: TITO was launched using: RESULT: Template: 1R5M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1774 3149 1.78 10.22 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 1.78 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.296

(partial model without unconserved sides chains): PDB file : Tito_1R5M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1R5M-query.scw PDB file : Tito_Scwrl_1R5M.pdb: