Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------------------------------------MSKKRRNRHKKEAQEPQFDFDEAKELTV---GQAIRKNEEVEAGVLPEDS-----ILD-KYVKQHRDEIEADKFATRQYKKEEFVETQSLDD--LIQEMREAVEKSEASSEEV-P-----SSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQED--S-EKKSKKGFILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTT----ANQSDVDDFNTLYDAFYTNSNK-----TALKNSQFDKLSQLKTLLDKLEG--------SREHTLAKSKYDSLATQIKAIQDVN-------------AQFEKPAIVDG-----VLDTNAKAKSDAKFTDI--------KTGNT--ELDKVLDKA-------ISLGKSQQTSTSSSSSSQTSSSSSSQASSNTTSEPKPSSSNETRSSRSEVNMGLSSAG------VAVQRSASRVAYNQSAIDDSNNSAWDFADGVLEQILATS----RSRGYITGDQYILERVNIV------NGNGYYNLYKPDGTY-------------LFTL-NCKTG------YFVGNGAGHADD-LDY-------------- 1YVU Chain:A ((4-706)) EALLNLYRIEYRPKDTTFTVFKPTHEIQKEKLNKVRWRVFLQTGLPTFRREDEFWCAGKVEKDTLYLTLSNGEIVELKRVGEEEFRGFQNERECQELFRDFLTKTKVKDKFISDFYKKFRDKITVQGKNRKIALIPEVNEKVLKSEEGYFLLHLDLKFRIQPFETLQTLLERNDFNPKRIRVKPIGIDFVGRVQDVFKAKEKGEEFFRLCMERSTHKSSKKAWEELLKNRELREKAFLVVLEKGYTYPATILKPVLTYERNEVADIVRMEPGKRLNLIRYILRRYVKALRDYGWYISPEEERAKGKLNFKDTVLDAKGKNTKVITNLRKFLELCRPFVKKDVLSVEIISVSVWRKEEFLKELINFLKNKGIKLKIKGKSLILAQTREEAKEKLIPVINKIKDVDLVIVFLEFLLYDFVKRELLKKMIPSQVILNRTLKNENLKFVLLNVAEQVLAKTGNIPYKLKEIEGKVDAFVGIDISRITRDGKTVNAVAFTKIFNSKGELVRYYLTSYP-AFGEKLTEKAIGDVFSLLEKLGFKKGSKIVVHRDG-RLYRDEVAAFKKYGELYGYSLELLEIIKRNNPRFFSNEKFIKGYFYKLSEDSVILATYNQVYEGTHQPIKVRKVYGELPVEVLCSQILSLTLMNYSSFQPIKLPATVHYSDKITKLMLRGIEPIKKEGDIMYWL

General information: TITO was launched using: RESULT: Template: 1YVU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1991 174621 87.71 377.97 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 87.71 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.028

(partial model without unconserved sides chains): PDB file : Tito_1YVU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YVU-query.scw PDB file : Tito_Scwrl_1YVU.pdb: