Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTALVVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVD-LASHYHFSMLAKNAKDMAAGAVLVVSLFAALTGALIFLPRIWDLLF 3ZE3 Chain:D ((23-125)) ------------------AGYSWKGLRAAWINEAAFRQEGVAVLLCVVIAAWLDVDAVTRVLLISSVMLVMIVELLNSAIEAVVDRIGSEYH--ELSGRAKDLGSAAVLIAIIDAVITWAILL---------

General information: TITO was launched using: RESULT: Template: 3ZE3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 251 -36389 -144.98 -356.75 target 2D structure prediction score : 0.80 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : -144.98 2D Compatibility (Sec. Struct. Predict.) : 0.80 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.292

(partial model without unconserved sides chains): PDB file : Tito_3ZE3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZE3-query.scw PDB file : Tito_Scwrl_3ZE3.pdb: