Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEEYIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQVSALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILYIETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRKNLEIVFYEDHRCPISRLKIRKLKDILEKKSQK 2B4A Chain:A ((14-131)) QPFRVTLVEDEPSHATLIQYHLNQLG------AEVTVHPSGSAFFQHRSQLSTCDLLIVSDQLVDLS--IFSLLDIVKEQTKQPSVLILTTGRL-----IESSEHNLSYLQKPFAISELRAAIDYHKPS---------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2B4A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 508 2934 5.78 25.74 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 5.78 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.572

(partial model without unconserved sides chains): PDB file : Tito_2B4A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B4A-query.scw PDB file : Tito_Scwrl_2B4A.pdb: