Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRNNSQNLIMLKHDFASHIEDLKCATKPRSEALGRHLWTCTID-ADFYWLKFHLPNVHAQSEQDFLHELQFYEDITYKKANWLLPFKIIEGRTISQQTQFQGKVLV--LPDTECWFDDLDQ---KQNLKNINEKIYLALVKLAELHELGWIHGDIKKEHFRKFK-QELYLIDFEKTRLISSP----DPITDATPRYMAPELFHGANKTVQSDLYALGIVLYEWLT-QTRLQANSYHEWAVLH-CQKLNVELPSS-FQIFLPLLSGLLQKQQQNRFSNVHEAINCLKALST 4Y95 Chain:C ((2-261)) ----------------WEIDPKDLTFLKELGTGQFGV-VKYGKWRGQYDVAIKMIKEGS--MSEDEFIEEAKVMMNLSH--EKLVQLYGVCT--------KQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGMTRFVLDDEYTSSTGTKFPVKWASPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLPRPHLASERVYAIMYSCWHEKADERPT-FKILLSNILDV--

General information: TITO was launched using: RESULT: Template: 4Y95.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1196 -16347 -13.67 -66.45 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.69 3D Compatibility (PKB) : -13.67 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.355

(partial model without unconserved sides chains): PDB file : Tito_4Y95.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y95-query.scw PDB file : Tito_Scwrl_4Y95.pdb: