Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHTSTTSTVPLEPWIAQQLQQATQGYWHKDQIPQSEIKRILTDSRHAESGDAFLALKGERFDAHNFVAQVAANGCQVAIVERPIDAEIAQLVVADTRLALGQLGAYRREQNAQLKVIALTGSSGKTTTKEMLGSILSRLAPTL-INRGNLNNDLGVPMMLLELRKEHQYAVMELGANHQGEIDYTSKIVQPHVAGILNIGTAHLGEFGGRDGICRAKSEIYRHILPQGVAIVPQQDDFTAEIREAAKSHQIMSFGAG--GDVFATEIELLPQSANFQLHTPQ-GSSFVRLPFAGEHNVQNATAAVAFALALGVSLEDIVKGLEQAQGAKGRLNFIQK----------APHLFIDDTYNANPTSMRAAAQVLLQ-QNGIKVMVMGDIGELGDSSWQEHHDLGRDLAGLPLDHIVAV-----GQ------FASAALEGAGLHS-TKLKAFQTQAEALPFLINLIQTHQPQSMSFLFKGSRFTHMETLMADLMEKL 4HV4 Chain:B ((122-480)) -----------------------------------------------------------------------------------------------------------------RHGIAVAGTHGKTTTTAMLSSIYAEAGLDPTFVNGG----LVKAAGTHARLGSSRYLIAEADESDAS-----FLHLQPMVAIVTNIEAD------DFENLKQTFINFLHNLPFYGRAVMCIDDPVVRELLPRV-GRHITTYGFSDDADVQIASYRQEGPQGHFTLRRQDKPLIEVTLNAPGRHNALNAAAAVAVATEEGIEDEDILRALVGFQGTGRRFDFLGNFPLAPVNGKEGSAMLVDD-YGHHPTEVDATIKAARAGWPDKRIVMLFQPHRYTRTR-DLYDDFANVLSQ--VDVLLMLDVYAAGEPPIPGADSRALCRTIRNRGKLDPILVPDSESAPEMLAQIL----NGEDLILVQGAGNIG--KIARKLAE--

General information: TITO was launched using: RESULT: Template: 4HV4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1751 -13580 -7.76 -41.78 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -7.76 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_4HV4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HV4-query.scw PDB file : Tito_Scwrl_4HV4.pdb: