Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQTLHVELGERRYPIFIGSQL--D-PKQ-LLEPYIHGQQVMIVSNVTVAPLYLSHYQEALESLGK------TVATCILPDGEKYKDIQHLNLIFDALLEAG--FNRDCTVLALGGGVIGDMAGFASACFQRGVYFVQVPTTLLSQVDSSVGGKTGINHPLGKNMLGAFQQPQVVLADMAQLNTLPERELSAGLAEVIKYALLGDEDFLVWLEENMDGLVAR--------------DADLLAEAVYRSCAHKARIVANDEKEQGERALLNLGHTFGHAIESYLGYGTWLHGEAVATGMVMAADLSQRLGWISNEDVARTKKIIQRANLPISCPQI-----------PLDDFLGHMAHDKKVLNGQLRLVLLKQLGQAVITK--DFDVELMKQAILANQHG 1SG6 Chain:B ((10-391)) ---------LGRESIIADFGLWRNYVAKDLISDC-SSTTYVLVTDTNIGSIYTPSFEEAFRKRAAEITPSPRLLIYNRPPGEVSKSRQTKADIEDWMLSQNPPCGRDTVVIALGGGVIGDLTGFVASTYMRGVRYVQVPTTLLAMVDSSIGGKTAIDTPLGKNLIGAIWQPTKIYIDLEFLETLPVREFINGMAEVIKTAAISSEEEFTALEENAETILKAVRREVTPGEHRFEGTEEILKARILASARHKAYVVSA----GGLRNLLNWGHSIGHAIEAILTP-QILHGECVAIGMVKEAELARHLGILKGVAVSRIVKCLAAYGLPTSLKDARIRKLTAGKHCSVDQLMFNMAL--------KKIVLLSAIGTPYETRASVVANEDIRVVL------

General information: TITO was launched using: RESULT: Template: 1SG6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1727 -32363 -18.74 -97.77 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -18.74 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.622

(partial model without unconserved sides chains): PDB file : Tito_1SG6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SG6-query.scw PDB file : Tito_Scwrl_1SG6.pdb: