Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLYASLFYQKDVTEIFSDSSLITYMIQVEVALAQAQAQVGV-IPQNAANTIAQVAEHALDKFDFSALAVATSLAGNIAIPFVKQLTAIVKDIDEDASRYVHWGATSQDILDTACILQCRDALNMVEGQLQQCYATALQQAKQYRHQVMIGRTWLQQALPITLGHKLARWASAFKRDLDRIQAMKSRVL-TAQLGGAVGSLASLQD----Q---GSLVVSAFAKQLNLTVP-TS-TWHGERDRIVEIASVLGMIVGNTGKMARDWSLMMQTEIAELFEPTAKGRGGSSTMPHKRNPVAAASVLAAANRVPALMSSIYQSM-VQEHERSLGAWHAEWLAVPEIFQLCAGALTRTGEVLQGFEVNAEHMQQNLECTNGLIMAEAVMMTLA-PKIGRLNAHHLVEAACKTAVAQNQHLFDVVSQLDEVKGQFSQEEIRNIFKPENYLGNIQQQIDAVLKEAQGESK 5EYV Chain:B ((25-480)) -RNPLTKRYASREMVCNFGEKRKVILWRQLWIWLAETQKELGFDITDEQINEMKSQR----DSVDFGTAAAEEKARRHDVMAHVYTFALACP----KAAPIIHLGATSCFVGDNADLIMLKDGLNILLPKVARCIDRLAKKAMLHKSLICLARTHLQPAQPTTMGRRICMWIQDLLLDLENLERLKNHTIRFRGAKGAVGTQASFMDLFQGDHQKVIKLDEILTKKSGFQRSWCVTGQTYPRKVDIEITNALSNIGATVHKICTDIRLLSS--FHEVEEPFE-----------KRNPIRSERACSLARYLMHISTSMVSTVSVQWLERSLDDSAIRRIVLPEAFLAADACLTLLQNIAEGLIVYPMVMEANLNSELPFLVVERILVKMVSEGAA----------------------------------------LPTVLDPSYMIGRAVEQVEVFLNTEVDP--

General information: TITO was launched using: RESULT: Template: 5EYV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1772 -63952 -36.09 -165.25 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -36.09 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_5EYV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EYV-query.scw PDB file : Tito_Scwrl_5EYV.pdb: