Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWKMDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDNERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVLLGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDT--THAFIEKVIFPKVKAG--AFSHEMDHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQAAAENALAAL 1RC7 Chain:A ((2-217)) ------KMLEQLEKKLGYTFKDKSLLEKALTHVSYSK------KEHYETLEFLGDALVNFFIVDLLVQYSPNKREGFLSPLKAYLISEEFFNLLAQKLELHKFIRIKRGK------INETIIGDVFKALWAAVYIDSGRDANFTRELFYKLFKEDILSAIKEGRVKKDYKTILQEITQKRWKERPEYRLISVEGPHHKKKFIVEAKIKE-YRTLGEGKSKKEAEQRAAEELIKLL

General information: TITO was launched using: RESULT: Template: 1RC7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 872 -37544 -43.06 -177.09 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -43.06 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_1RC7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RC7-query.scw PDB file : Tito_Scwrl_1RC7.pdb: