Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRFAINGYGEAQDVFEEIDAHPR-EVGSGHVRVEIKAFSVNPYDVALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAV---GGTYGEEVVLPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQ-----PTDTVMILGASGAVGSSLIQLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNP--GLQF-ADQADLVIDATKGSIKGETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYE-SFVPRK--EYLDAEAFAGIIKAYQKGAFHVFISMNLSASLKHVIQAHQLVEG-HPPAGKIILSFEK 5A4D Chain:A ((6-329)) MHALQYNSYGGGAAGLEHVQV-PVPTPKSNEVCLKLEATSLNPVDWKIQKGMIRPFLPRKFPCIPATDVAGEVVEVGSGVKNFKAGDKVVAVLSHLGGGGLAEFAVATEKLTVKRPQEVGAAEAAALPVAGLTALQALTNPAGLKLDGTGKKANILVTAASGGVGHYAVQLAKLANAHVTATCGARNIEFVKSLGADEVLDYKTPEGAALKSPSGKKYDAVVHCANGIP-FSVFEPNLSENGKVIDITPGPNAMWTYAVKKITMSKKQLVPLLLIPKAENLEFMVNLVKEGKVKTVIDSKH--PLSKAEDAWAKSIDGH-ATGKIIVEP--

General information: TITO was launched using: RESULT: Template: 5A4D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1802 29566 16.41 95.99 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 16.41 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_5A4D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5A4D-query.scw PDB file : Tito_Scwrl_5A4D.pdb: