Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPGPRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPYEPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESG-WGDMAYYFEHEEEPDFGFSPDA-EFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVPGTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQSGINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAGLFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVME-PHYVPMDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEFMSLDDLFRATAIYADAIYRLAK 2F8H Chain:A ((0-365)) -------HMTDLLASTLEHLETLVSFDT------RNPPRAIAA-EGGIFDYLRAQLPGFQVEVIDHGDGAVSLYAVRGTPKYLFNVHLDTVPDSPHWSADPHVMRRTEDRVIGLGVCDIKGAAAALVAAAN-------AGDGDAAFLFSSDEEANDPRCIAAFLARGLPYDAVLVAEPTMSEAVLAHRGISSVLMRFAGRAGDP-----------------------AASALHQAMRWGGKALDHVESLAHARFGGLTGLRFNIGRVDGGI---------------------------------------------------------------------KANMIAPAAELRFGFRPLPSMDVDGLLATFAGFADPAAAHFEETFRGPSLPSGDIARAEERRLAARDVADALDLPIGNAVDFWTEASLFSAGGYTALVYGPGDIAQAHTADEFVTLAQLQRYVESVNRIINGS--

General information: TITO was launched using: RESULT: Template: 2F8H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2100 -65546 -31.21 -183.60 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -31.21 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.463

(partial model without unconserved sides chains): PDB file : Tito_2F8H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F8H-query.scw PDB file : Tito_Scwrl_2F8H.pdb: