Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------------------------------------------------------------------MKTRRKPMKFMTLNTHSWLEPE--PEKKLQELADKILLE-DYEVIALQEINQLLESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLV-----QLLKEHGKEYFWSWEMSHIGYDKYEE--GNALLTKKTLE-SQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKSGFYFEWKNLENYFLETR---------VPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKAATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVNE--------------------------------------------------- 4Z0V Chain:A ((7-440)) PPAFTELQLPRYIMAGFPVCPKLSLEFGDPASSLFRWYKEAKPGAAGSSWTETDVEERVYTPSNADIGLRLKLHCTPGDGQRFGHSRELESVCVVEAGPGTCTFDHRHLYTKKVTEDALIRTVSYNILADTYAQTEFSRTVLYPYCAPYALELDYRQNLIQKELTGYNADVICLQEVDRAVFSDSLVPA--LEAFGLEGVFRIKQHEGLATFYRKSKFSLLSQHDISFYEALESDPLHKELLEKLVLYPSAQEKVLQRSSVLQVSVLQSTKD-------------SSKRICVANTHLYWH---PKGGYIRLIQMAVALAHIRHVSCDLYPGIPVIFCGDFNSTPSTGMYHFVING------------------SIPEDHEDWASNGEEERCN-------MSLTHFFKLKSACGEPAYTNYVG-----GFHGCLDYIFIDLNALEVEQVIPLPSHEEVTTHQALPSVSHPSDHIALVCDLKWK

General information: TITO was launched using: RESULT: Template: 4Z0V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1087 34876 32.08 147.78 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 32.08 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.226

(partial model without unconserved sides chains): PDB file : Tito_4Z0V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z0V-query.scw PDB file : Tito_Scwrl_4Z0V.pdb: