Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFNMKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHYLEG-KNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMFDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVAYCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDDVDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLPAFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNLFIPRV 2I6U Chain:C ((3-300)) -----------RHFLRDDDLSPAEQAEVLELA---AELKKDPVSRRPLQGPRGVAVIFDKNSTRTRFSFELGIAQLGGHAVVVDSGSTQLGRDETLQDTAKVLSRYVDAIVWRTFGQERLDAMASVATVPVINALSDEFHPCQVLADLQTIAERKGALRGLRLSYFGDGANNMAHSLLLGGVTAGIHVTVAAPEGFLPDPSVRAAAERRAQDTGASVTVTADAHAAAAGADVLVTDTWTSMGQENDGLDRVKPFRPFQLNSRLLALADSD--AIVLHCLPAHRGD-----------------EITDAVMDGPASAVWDEAENRLHAQKALL------------

General information: TITO was launched using: RESULT: Template: 2I6U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1753 -12181 -6.95 -41.01 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain C : 0.80 3D Compatibility (PKB) : -6.95 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.584

(partial model without unconserved sides chains): PDB file : Tito_2I6U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I6U-query.scw PDB file : Tito_Scwrl_2I6U.pdb: