Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGYRHIDTAQGYKNEESVGQAIKES----GIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQTDYLDLFLIHWPNPVAFRDNW----EQANA--------DTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAKTA--KIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTG----------KIFDVPEMKQIAEAHDKTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAKDPDTTQF 4JII Chain:X ((9-291)) ---------LSTKAKMPIVGLGTWKSPLGKVK-EAVKVAIDAGYRHIDCAYVYQNEHEVGEAIQEKIQEKAVKREDLFIVSKLWPTFFERPLVRKAFEKTLKDLKLSYLDVYLIHWPQGFKSGDDLFPKDDKGNAIGGKATFLDAWEAMEELVDEGLVKALGVSNFSHFQIEKLLNKPGLKYKPVTNQVECHPYLTQEKLIQYCHSKGITVTAYSPLGSPDRPWAKPEDPSLLEDPKIKEIAAKHKKTAAQVLIRFHIQRNVIVIPKSVTPARIVENIQVFDFKLSDEEMATI------------------

General information: TITO was launched using: RESULT: Template: 4JII.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 1269 13402 10.56 52.55 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain X : 0.80 3D Compatibility (PKB) : 10.56 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.548

(partial model without unconserved sides chains): PDB file : Tito_4JII.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JII-query.scw PDB file : Tito_Scwrl_4JII.pdb: