Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDEVINVISKCITQLNLFVDGPAAHLSGTAAPTTIETIRKCIADHAYLFTDHRINGQRMWELVCSATFTVGEPRTPTTAATATATLLSLFRRLTDSVAASARAPEADILKHLVKLLRDAVKPPLVRGASPGQLRCLFQAAMRLLKLLLPLKKRADVQTECLHLLLALMQLSDLQEAHCAVVASGDEVAQVAKLCADICGAKHDVALRPQNASMQLLGCLCEQPAYVHALSGQLAERILKCFLDVARNLAQRAEVNQTLGEGLLRGLAGVLGSFPLRCEEDAPALTLINNIVLSALMLGETSTNYTFCLSALFLLRRRASDTLAPFTLLCAAEYSVALRSLWMHRNKDVRRETHAAAAVFWSALSSQLQQRCSSEVADANTTRNTAASHKLHSIVEFLLRSLDSSADPEASYALEALYHLLPAVAALSGPQALQTMSTRLEERVGGLLITASAQRTEALFQQVPYLLATLGQLLTLLPSVSPRQQAVVRELLDWVLLLYPHPKFYNREKTVPGIVLVIRALTHHPRAPHNTLPRLLSERTLELVGEAPGAAMMRLAGERISTEERLDRMATLWTRLLTTREESGAEAEAESKQRLSAAFYRACTGFCERARLEVIVKSNSEATLERILVDGVDGVQPSFPTHYSAFLSFVDFFVRLSEGLKGSGGVCRPPAEELTAWLEALVRGASAALHVSGFYTLLHRTVVGVEGVCPHSLTHQRPYTDASLASGNSDVTSDGHHENHLCVSPLLRHFLSTECLRRVHVYSDELLYQCAMCVVVGAALVGSEDRGELAGSRARACDDHRRLDAYVRAFKVVMTATPTSLQAESVAGSTNEVVCSFGSSPAILESNRQHALRVLEKKACRSPRWAAAVLPALTSALRSAEVVAAVRAPVQMLAARHGVAIAAELQHHSNEATFALLCRGEPASNGRRDSMPRWLATMQPVTVPLVPVDSALHIGYLVPLVSRLSLYGKHPAVRTAAAEVLYWCVVWVIGKKVNDWGSAVFPPLLSLASLARTSTGGSGGGGGSMVHQELHGLLMQAARWFGRSAGTPEEAELFVSVLLRGLGEREGTQRQVATEALFAYAIPPADEPQTVTPHFRAVIGRLVRMECGVSEWSRLGAAHCLHVMMRRLCKVRVPGLVCTVQAVAQGAVGCLRRCTEAPIWGAMETATCNEVQESLRWLGAYCHDIRQLENEEEQREADRFAADLVHAARAVLPALARRHTVCAITLLQTLPAVTHTNASARQVLLLSQELICEQQQRLGPAAVRSLDSTVGGCATLVALLQSGYGTETNKRAIAEAVTPVICTRADDCSVETELLRLLTVVLQAIVEEARVATHESRCISPHIVRWLLELVDVVPEQTHATHFSLLLTPTVIAQAARVMVAGAKVTVENCAGDVGEDATAGVPLRRCAPSSQLAPTARWCLELIEFLMRVAGQRRRGAQVIEDVLCAIRETSVFTVFTAASPARQLIDALSVGPSALVTGPVALAAALCQMEAPCSSGTTTLWMRIADSMKISPKTVVAMLLGLLRNSAALNATGGEEVCEGLLVLMLSEPDPALLRQHLRDLLASDAVQHNIFTSSSFAVSLEDGHGAVAVSPSSRSTSVLLSRAFDSVMNRCWQQASAALPTDRLVSALPSLTTVAMSEDALRSSSEAVSLFSLVGDWLAELQRRSLTSEHDKVLCRVCANLVAAEREMRQSTPTAAQDDGRGGARLAALLMLVQLIGPAIRTDTTLSSALCDLVLFVSKPANMCSLLASVDATEHVVWGFHVLAATMTPRPARRSLAVFPVDLRAVEMAFDFLNEVLNSALPLRWTELKTPREKTNGADVVRSGTALFAAVLPHKVQVLEVIAPLLAQESMPQRDTIVRQVHGALQALSVCQQALQLDACRCASRLLRDPRTHPGICDALTEHVLLPLLQASAEAVRLRLFEEEVRNWVTEAGRAPALGCFYMQTAAFSCLALMHRLCPLTELRGSISTAFTGRNPNTTGVELTLEVLKACRAAWQSPNTGGDDNSAEEADGDVGDSACADAPRRYRQAAFRCLLATLSQTQQAEKVFCRCFFQERSVAGWNGLFLVKRPGVAVTDLLDAVGEVQLWRDMDRCQVPELFAHLLRHFPSSSVTPGEPPEWVRHVVAVLRQPQLRLPVRMVFYEMICRHERFFGTFARCIFDGVADAVAAMAPSERAGKVIWELLALLTRWHREGSSRALAVGSAASLKALARYAAVHLAWEHSFTACEGSRALDPTSHKRLAELLEQFRRAQGAALAIRSTSQPLTAAEMKMLLSHPAYERHAALEVIYLITNACGCLGCTGDSREVVEVYDLLGAFLGASASPQLYHITARLVGLEHRLLSEAATREPSSAVRKLLRDALRGTVCAILSVCKSDKKFLEVLEAMQEDRSGDLVYQLLSRTDLLRRYSIRKDSEKLLTLRVLARAGTYVADNYDSIHVCLSGLLRGGQGEPLLGICTVVKNALPHMSMESVRHVLRDLCCLDVLQAMESAEPARIAFYKMGLAALRRWTVVREDQEKQDGGGSVSADAEAFLVRRGLRDASPAVQGAVLEYLDVHAQHVPDTMWGRLTCLFTAEDGASGDGHSGSGGWVRHVALLLLSLSKRSRDFANPTYFCPEALHSEGASPTLATSADTAAESSVAQRQPRSAPLFSEAVQCGSPAEAATANAACPITAALAQIYVPPALVPQPPHQQQQPLSGARRPRATGAWRLGAAPCAAATAVTRSWLRLESGDSEQENAVISAGVMASRQISATMQRNAAHGAALQAGRSVMNAPSAAPSLESNTAGRLSEVRLTLQSLLVPLQVLCLHEDEVAARVLIDLISNAAVASPVTSESPHIVSLCSALTSLLPKFQSGATTRQCELAKNPFTLFVALELLLHAQAAGWSSLASLDCAALVRLTSDVQAYGGSVPTAFLPVVDGTAHVVEKLLSSLQPSAARGVPTRPSLEFPALGWHAYHVAQRLRRHDDAACQAALQLFRATRRHEAPVIQLQGALDAMEVGGAAYSMRVIAKLLEDEEKEAAAAVATVASPATHVSGGAFALFAAREEVLRHYRSRASRLLLDWCGAASALQSATPVGAGGSGEVSVEGVRLRLRAQLQLMCNGAASAVTAEASTASRHRTGFEWGACLMANGRWLDCQLHAETSVDHLLESTASHYDSGGSSLVCGYLAANSLSQLADAAQVLRFVSTSAASSAGVALQTYLDTMPALVPLSGAYGSLFIDDVLLIRRLIIDYHVQSTGDLLGSLQFTDNERHKLVAQIHELLG----------TWTVQQCSELLLLPEKTSAIETIASKADHATLSEPTRCALELLKK------------RSSLFSIAVTPREASGASREEALAA-------FQKATGKDVQQRAAAGLLSPAAARQWAADVAQCEVEIFASIGSILSIP---AGSAASVQKREIWVSTLKENLRDTIPSHVALLNALEKLLPPVSRGD--GTLADMGGDAKDAARASYITAATQSLWQLYANTFLWCAQAVGDRSMQVGSGGHLPVSARVPRLLSLFALPRVAEAAAQAQWRGTWEALVMLPTLVWLPWATLLVNALLQTEHAVLARILLRLCRERGQVLYYPINCAWRSLADLGKRRNAGRAVDDDLTRELAELHKWHRSHRMALVAEFAAALDELVEPQHRLEVRLSEVESLVKQHEQCTRGGGGAQAEYLMAALQLYERCKSDLMDPARWGRAQLYHRRAAQVLQELDRRNAFSAEVLKSGEQFRSAKREALRQVAQTLSTLSTSASNSATRSQELRLYSERLQHALGRSATAATTTTTQLGYTSDAAEPILQPVQPTHLLHTPPDAAQQCCTLVGVADRVLVLKSKRLPKKVDIYTS------AGICTYMVKGGEDLRLDQRIQEVFTFANLCLLVSPRSRLSALTIRTYAVVPVSPLVGLICWVPRTTPLQELMKEATPPDAHDAVARFCRARGKPQEILPLYKTAHITGIPEQEYKDLVYCLRQSALVESLQSIATDHCSWLGARDAFLDTNAAASMASFVLGVGDRHAGNLLVDLLNSELVAIGFGLAFGDATRKLPVLELMPFRHTPQLQRVQGVLGDDVT------RCRMRHVLHVLRAERQVVEGMVAALLMPDADAIGDEESRIAGHQLDVVRRKLNLEHPAGIILRDVARNAYVTQDKDVWIGVKACLCERKRDENGEDADLTETALSALHQEDVFVQRLLRIASDPRVLGRAYAGWQAYM 1E8X Chain:A ((1-951)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AASEETLAFQRQLNALIGYDVTDVSNVHDDELEFTRRRLVTPRMAEVAGRDPKLYAMHPWVTSKPLPEYLLKKITNNCVFIVIHRSTTSQTIKVSADDTPGTILQSFFTKMANERDFVLRVCGRDEYLVGETPIKNFQWVRQCLKNGEEIHLVLDTPPDPALDEVRKECDRKFRVKIRGIDIPVLPRTADLTVFVEANIQYGQQVLCQRRTSPKPFTEEVLWNVWLEFSIKIKDLPKGALLNLQIYCQLLYYVNLLLIDHRFLLRHGEYVLHMWQLSGFNADKLTSATNPDKENSMSISILLDNHPIALEMPNQLRKQLEAIIATDPLNPLTAEDKELLWHFRYESLKDPKAYPKLFSSVKWGQQEIVAKTYQLLAKREVWDQSALDVGLTMQLLDCNFSDENVRAIAVQKLESLEDDDVLHYLLQLVQAVKFEPYHDSALARFLLKRGLRN-----KRIGHFLFWFLRSEIAQSRHYQQRFAVILEAYLRGCGTAMLHDFTQQVQVIDMLQKVTIDIKSLSAEKYDVSSQVISQLKQKLENLQNL--------NLPQSFRVPYDPGLKAGALV------------IEKCKVMASKKKPLWLEFKCADPTALSNETIGIIFKHGDDLRQDMLILQILRIMESIWETESLD----LCLLPYGCISTGDKIGMIEIVKDATTIAKIQQSTVGNTGA---------------------------------------FKDEVLSHWLKEKCPIEEKFQAAVERFVYSCAGYCVATFVLGIGDRHNDNIMISE-TGNLFHIDFGH--------------VPFVLTPDFLFVMGTSGKKTSLHFQKFQDVCVKAYLALRHHTNLLIILFSMMLMTGMPQLTSKED------IEYIRDALTVGKSEEDAKKYFLDQIEVCRDKGWTVQFNWFLHLVL-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1E8X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4140 -12461 -3.01 -15.67 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : -3.01 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.224

(partial model without unconserved sides chains): PDB file : Tito_1E8X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E8X-query.scw PDB file : Tito_Scwrl_1E8X.pdb: