Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSITLLQPDDWHAHLRDG-------LALKR-TVPDLAKQFARAICMPNLVPPVKTVEEALAYRERILAHVPEGNNFDPRMVLYFTDHT--SPDEVRKIKE-SEHVNAIKLYPAGATTNSNNGVSDIRKVYAVIEQLEEHQVPLLLHGEVTHNHV--------------------DIFDREKRFLDEVLSPLLKQFPKLKVVLEHITTSDAAHFVLEQD---RNVAATITPQHLLFNRNDMLVG--GIKPYFYCLPILKRQTHQTTLLEVATSGNPKFFLGTDSAPHAQNAKEN-----ACGCAGCYSAPNAIELYAQAF--DQVGKLERLEGFASHFGADFYGLPRNTSTITLVKE-DNLVPESFDYLDNQKIIPLHAGKTLQWRKV 5E5C Chain:A ((49-395)) --QYLMPGGIDPHTHMQLPFMGTVASEDFFSGTAAGLAGGTTSIIDFVIPNPRQSLLEAFHTWRGWA-QKS--AADYGFHVA--ITWWSDEVAREMGELVAQHG-VNSF-HFMAYKNA----IMAADDTLVASFERCLELGAVPTVHAENGELVFHLQQKLLAQGLTGPEAHPLSRPPQVEGEAASRA-IRIAET-LGTPLYLVHISSREALDEIAYARAKGQPVYGEVLAGHLLLDDSVYRHPDWATAAGYVMSPPFRPVEHQEALWRGLQSGNLH-TTATDHCCFCAEQKAMGRDDFSKIPNGTAGIEDRMALLWDAGVNSGRLSMHEFVALTSTNTAKIFNLFPRKGAIRVGADADLVL--------------------------

General information: TITO was launched using: RESULT: Template: 5E5C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1743 21441 12.30 71.00 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 12.30 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.312

(partial model without unconserved sides chains): PDB file : Tito_5E5C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5E5C-query.scw PDB file : Tito_Scwrl_5E5C.pdb: