Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSAIKPRSTKKLSQIIVEQLIEKIQSGEFKVGEKIPTELELIETFEVSRSVIREAITELRSLGFVETRHGIGTFVKEQTVEQNFLLSNASLETINDIISLLELRISLESEAVFLASERRKQIHIDKMKAALEDFERHISSDANDGTVKADYDFHIAIAEASENQYFVDFLKYLGEKIIPRARVKSIEQSPENREEYLKAVHHDHVNIYNAIVDQDGLLARQMMRAHLSKSIKKFKQ 3C7J Chain:B ((18-225)) ---GNEQPPAHLARTVIEEKLRNAIIDGSLPSGTALR-QQELATLFGVSRMPVREALRQLEAQSLLRVETHKGAVVAP--------------LITEDAVDAYALRILLESEALRLSIPLLDADDLAAAASYIEQLEVE-TDFG--QIGRLNRMFHLSLYAKTHNKRLMRLVEEGLNEEERFLR-FN-----LSSM-----SQDDHWQLLRLAEQKAVEPCVEALQYHLNRGVQAV--

General information: TITO was launched using: RESULT: Template: 3C7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 745 -87009 -116.79 -428.62 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -116.79 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.591

(partial model without unconserved sides chains): PDB file : Tito_3C7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C7J-query.scw PDB file : Tito_Scwrl_3C7J.pdb: