Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------MSQASPVYQGSCLCQGIRYEI--------------KGDIGDIIQCH--CQRCRKSNGTAFATNAPINSADFKITQGEDLIKKFAVNGVYRW--FCSECGSPLISSRDAQP--ELYRLRIGTLDTPLDQKPTMHIFAASKAEWECIHDDLPQYDERP-- 1QF6 Chain:A ((63-242)) KDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGFYYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRG-ESYKVSILDENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRIYGTAWADKKALNAYLQRLEEAAKR

General information: TITO was launched using: RESULT: Template: 1QF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 553 -3651 -6.60 -27.04 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -6.60 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.083

(partial model without unconserved sides chains): PDB file : Tito_1QF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QF6-query.scw PDB file : Tito_Scwrl_1QF6.pdb: