Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNATLNYQPHWVREDFVDFVLQKINATWAWKRVLAEVTAVQSLHTDMVLIKLKPNRNFNFDQVRAGESILLTLLIDG--VYQQRSYSIIEVTTQGEIALGIKVQ--GLVSRAAQL-LHVGECVEISQPQGDFTLHQG-QQPAILIASGSGVTAIYSLLQQALKQQL-EQIHVIYFNRA---EIFHAELKALAEQYPQLQYHFFNTTEQK-----QHLTE------SLLQKLVPDFEQTATYVCGHHGMMQQANEIYTQKGAQ-SQLHQEYFQPLQVTGTHAAQPVIFRRAQQEFLAETNLLGSAEQAGLRPQHGCRMGVCNKCSCTKVSGVTQNLLTGEIEDQPNRPIKLCVSQALSPVTIDL 1GVH Chain:A ((148-394)) --------------------------GWE-GTRDFRIVAKTPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYRIAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLAMLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAKGQFDSEGLMDLSKLEGA-FSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGPHK----------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1GVH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1195 -12643 -10.58 -56.19 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -10.58 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_1GVH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GVH-query.scw PDB file : Tito_Scwrl_1GVH.pdb: