Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHSDTLSLSLELLQQPSVTPID---HTCQTIMADRLAKVGFHIEPMRFG---DVDNLWARR-GTEGPVFCFAGHTDVVPTGRLDAWNSDPFAPEIRDGKLYGRGSADMKTALAAMVVASERFVAKHPNHKGSIAFLITSDEEGPAVNGTVKVIETLEKRNEKITWCLVGEPSSTH--KLGDIVKNGRRGSLNAVLKVQGKQGHVAY-PHLARNPIHEASPALAELCQTVWDNGNEYFPATSFQISNIHAGTGATNVIPGTLEVTFNFRYSTEVTAEQLKQRVHEILDKH---GLQYEIVWNLSGLPFLTPV-G-ELVNAAQTAILNVTGTETELSTSGGTSDGRFIAPTGAQVL-ELGVLNATIH-QINEHVDVHDLDPLTDIYEQILENLLA 1CG2 Chain:C ((17-390)) -EQPAVIKTLEKLVNIETGTGDAEGIAAAGNFLEAELKNLGFTVTRSKSAGLVVGDNIVGKIKGRGGKNLLLMSHMDTVYLK--GILAKAPFR--VEGDKAYGPGIADDKGGNAVILHTLKLLKEYGVRDYGTITVLFNTDEEKG-SFGSRDLIQEE---AKLADYVLSFEPTSAGDEKL----SLGTSGIAYVQVNITGKASHAGAAPELGVNALVEASDLVLRTMNIDDK-----AKNLRFNWTIAKAGN-VSNIIPASATLNADVRYARNEDFDAAMKTLEERAQQKKLPEADVKVIVTRGRPAFNAGEGGKKLVDKAVAYYKEAGGTLGVEERTGGGTDAAYAALSGKPVIESLGLPGFGYHSDKAEYVDISAIPRRLYMAARLIMDLG-

General information: TITO was launched using: RESULT: Template: 1CG2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1989 56060 28.19 157.03 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.71 3D Compatibility (PKB) : 28.19 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_1CG2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CG2-query.scw PDB file : Tito_Scwrl_1CG2.pdb: