Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYTFDYLVFIGRFQPFHLAHMQTIEIALQQSRYVILALGSAQMERNIKNPFLAIEREQMILSNFSLDEQKRIRFVHVVDVYNDEKWVKQVKSLINGVIEPNSKVGLIGHFKDESS--------YYLRLFPEWVMVELD--SLKDSISATPMREAYYQGKIKTDAFPKGTIQFLEEFQNTPLYLQLQQKFLQADHSNIEETVSSHKE 5H7X Chain:E ((8-168)) HMSKTRVIYPGTFDPITNGHVDLVTRASRMFDEVVVAIAIGH---HKNPLFSLEERVALAQSSLGHLS--NVEFVGFDGL-----LVNFFKEQ-------KATAVLRGLRAVSDFEYEFQLANMNRQLDPHFEAVFLTPSEQYSFISSTLIREIARLKGDVTKFVPQAVVEAFERKHQ----------------------------

General information: TITO was launched using: RESULT: Template: 5H7X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 677 -67930 -100.34 -449.87 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain E : 0.64 3D Compatibility (PKB) : -100.34 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_5H7X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5H7X-query.scw PDB file : Tito_Scwrl_5H7X.pdb: