Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDK-------TLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK 3TNY Chain:A ((17-296)) -------------------------------------EVVTVEHAMGKTEVPANPKRVVILTNEGTEALLELGVKPVGAVKSWTG----DPWYPHI---KDKMKDVKVVGDE-GQVNVETIASLKPDLIIGNKMR-HEKVYEQLKAIAPTVFSET--LRGEWKDNFKFYAKALNKEKEGQKVVADYESRMKDLKGKLGDKV---NQEISM-VRFM---PGDVRIYHGDTFSGVILKELGFKRPGDQNKD---DFAERNVSKERISAMDGDVLFYFTFDKGNEKKGSELEKEYINDPLFKNLNAVKNGKAYKVDDV--IWN-TAGGVIAANLLLDDIEKRFV-------

General information: TITO was launched using: RESULT: Template: 3TNY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1480 -76260 -51.53 -279.34 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -51.53 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_3TNY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TNY-query.scw PDB file : Tito_Scwrl_3TNY.pdb: