Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFSPLCWIVPSIYVIMKEGFKIGKGVFMLKLGVIGTGAISHHFIEAAHTSG--EYQLVAIYSRKLETAATFASRYQNIQLFDQLEVFFKS-SFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVSQPQEWFDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLAD---KQVLGADFNYAKYSSKMPDLLAGQTPNVFSDRFAGGALMDLGIYPLYAAVRLFGK--ANDATYHAQQLDNSIDLNGDGILFYPD-YQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEH-IRSAIFTDHQGNQV---QL--PIQQAPHTMTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK 2POQ Chain:X ((2-332)) ----------------------------ALRWGIVSVGLISSDFTAVLQTLPRSEHQVVAVAARDLSRAKEFAQKHDIPKAYGSYEELAKDPNVEVAYVGTQHPQHKAAVMLCLAAGKAVLCEKPMGVNAAEVREMVTEARSRGLFLMEAIWTRFFPASEALRSVLAQGTLGDLRVARAEFGKNLTH--------VPRAVDWAQAGGALLDLGIYCVQFISMVFGGQKPEKISVMGRRHETGVDDTVTVLLQYPGEVHGSFTCSITAQLSNTASVSGTKGMAQLLNPCWCPTELVVKGEHKEFLLPPVPKNCNFDNGAGMSYEAKHVRECLRKGLKESPVIPLVESELLADILEEVRRAIGVTFPQD-

General information: TITO was launched using: RESULT: Template: 2POQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 1854 3924 2.12 12.42 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain X : 0.68 3D Compatibility (PKB) : 2.12 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_2POQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2POQ-query.scw PDB file : Tito_Scwrl_2POQ.pdb: