Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALIRKRRLTEQQQRRIEKQHKTRQEEVDTSQDLDGLVVQHYGRQLEVQALSVPAHHPEKPQVAEGEPEPFWKPIELNSVWRCHTRTN---LELLVTGDRVKWQADPNTGLGIITAIHPRTSLLTRPDRYHKVKPVAANISLIVIVFA-PLPEPAPTLIDRYLVACADANIPALLVLNKSDLLTEN--NPILDMLKEYENLGYEVMICHS-KGD-ISALSQRLDGETVAFVGQSGVGKSSLINVLIPDAEQKTNIISENSALGQHTTTSTRLINFGRNGALIDSPGIREFGL-WHLDLDKIRMGFPEIEAHLGSCQFRNCTHTHEKNCGLKQAVEAGEILPRRLDSFLRLIDEIQEAQQKN 2YV5 Chain:A ((1-297)) ---------------------------MGKKELKRGLVVDREAQMIGVYLFED------------------------GKTYRGIPRGKVLKKTKIYAGDYVWGEVVD-PNTFAIEEVEERKNLLIRPK--------VANVDRVIIVETLKMPEFNNYLLDNMLVVYEYFKVEPVIVFNKIDLLNEEEKKELERWISIYRDAGYDVLKVSAKTGEGIDELVDYLEGFICILAGPSGVGKSSILSRLTGEE-LRTQEV---------TTTGVRLIPFGKGSFVGDTPGFSKVEATMFVKPREVRNYFREFLR--YQCKYPDCTHTNEPGCAVKEAVKNGEISCERYKSYLKIIKVYLEEIKE-

General information: TITO was launched using: RESULT: Template: 2YV5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1450 12549 8.65 44.98 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 8.65 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_2YV5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YV5-query.scw PDB file : Tito_Scwrl_2YV5.pdb: