Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAWQNVEVVHVVAMDKNHCIGKGNALPWHISADLKHFKEITQGGVVIMGRKTLESMGRALPNRVNWVITRDINWHFDGVKIAYSIEDALNAALEDAKNTEKQALFIIGGGEIFKQTLSIADRLELTHVDLDVQGDAHYPTIPSEFHKTASEQQ--VDEKSGTSFEFATYKK 4ELG Chain:C ((3-160)) -------VSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEE----VFELCKNEEE--IFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEK

General information: TITO was launched using: RESULT: Template: 4ELG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 740 -103640 -140.05 -664.36 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : -140.05 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.600

(partial model without unconserved sides chains): PDB file : Tito_4ELG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ELG-query.scw PDB file : Tito_Scwrl_4ELG.pdb: