Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLNFRMIPKDQYPSDVKILRVVSINVNGLRSSVTKGLLEWLEQSDADVVCMQESRITHEQWTEKFRPEGWHTHLFPAERAGYAGTAIYSRLPFVSIKDGLGFELADSQGRFISAEFDLGLSHPVHIASLYLPSGSSGEEAQARKDLFLGEYAKILKQWRDENKSIIICGDYNIVHKRIDIKNWSGNQKSSGCLPHERAWLDHIYDELGYVDTFRVVRTEAELYSWWSNRGQARAKNVGWRIDYHACSPDWKARTVNAWVYKDQWFSDHAPVIIDYKIQE 5EWT Chain:A ((1-247)) -------------------MKIVSWNVNGIRAALKKNLIDFIENNMFEVIMFQETKGDIVPLDF--IMMGYEVISFPAKRKGYSGVMTLTKIKPINVIKGLQIKEFDDEGRTVTLE-----LKDFYVINAAFPRAGDNLERLDFKLKFNNEIENFVLKLR-RAKPVILCGDFNIAHQNIDGAFSDPT--IPGLTPQERSWFSHF-LSLGFIDTFRYLHPNVRKYSWWSYMGKAREKNLGLRLDYCIVSEELKDRIKMADILIDIQGSDHAPIILELT---

General information: TITO was launched using: RESULT: Template: 5EWT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1394 -152989 -109.75 -619.39 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -109.75 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.453

(partial model without unconserved sides chains): PDB file : Tito_5EWT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EWT-query.scw PDB file : Tito_Scwrl_5EWT.pdb: