Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIVLANPRGFCAGVDRAIAIVNRALECFNPPIYVRHEVVHNKFVVDDLRQRGAVFVDELDQVPDDSIVIFSAHGVSKAVQQEAERRGLKVFDATCPLVTKVHIEVTKYAREGTEAILIGHEGHPEVEGTMGQYDKLKGGDIYLVEDEADVAALEVRHPEKLAFVTQTTLSIDDTAKVIDALRAKFPNIQGPRKDDICYATQNRQDAVRDLAEKCDVVLVVGSPNSSNSNRLRELAERMGKAAYLVDNADQLEQSWFNDTCKIGVTAGASAPEILIKQVIQRLQDWGAQAPKELEGREENITFSLPKELRIHVTQA 3T0G Chain:B ((13-322)) MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRYVVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKVHMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGG-MYLVESPDDVWKLTVKNEEKLSFMTQCTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVLVVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQNVVARLQQLGGGEAIPLEGREENIVFEVPKELRV-----

General information: TITO was launched using: RESULT: Template: 3T0G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1791 -192458 -107.46 -620.83 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.93 3D Compatibility (PKB) : -107.46 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.590

(partial model without unconserved sides chains): PDB file : Tito_3T0G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T0G-query.scw PDB file : Tito_Scwrl_3T0G.pdb: