Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVQPNDYSKIYDIAVIGGGINGVGIANDAVGRGLSVFLCEKDDLASHTSSASSKLIHGGLRYLEYKEFRLVREALAEREVLLAKAPHIIKPMRFIMPHRPHLR----PAWLIRAGLFFYDHLGKREKL-LGSNRIYFKE----DSPLKP-AITRGFEYSDCTVDDARLVVLNALQAKEKGAKVVTRKRCVKAYRQ-QELWYLELQS--GAEFYQVRAKAIVNAAGPWVEEIISENLNLSSPYQIRLIQGSHIVVPKLYD-CHKAFIMQN--EDRRIVFAIPYLEKYTLIGTTDQEYTGDPQKVEITDVEIDYLLTVTNSHFKK-QLTRADIVSQYSGVRALCDDESDNPSAITRDYTLALQAEDKTTPLLSVFGGKITTYRKLAEAALEHLAPFFNDM-----AEEWTADDPLPGA-ENWTTLEDL---INQIKTRVSGISDSLANRWAHAYGTRVWNMLKERNAIEQLGQHFG--HDLFECEVRYLCEYEWAHTAEDILWRRSKLGLAFDE-KQ---VKVLEAYLSERRLKDDAA 2RGH Chain:A ((28-546)) ------MQQEELDLLIIGGGITGAGVAVQAAASGIKTGLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQGIAPHIPKPDPMLLPIYEDEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGLKGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYEGDQIVGVKARDLLTDEVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFDTGKQDGRMVFAIPREN-KTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANITLADIEASWAGLRPLLIG-----------SSLERE----PDGLLTLSGGKITDYRKMAEGALRLIRQLLKEEYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAA-GLEEEDATYIADFYGTNARRIFELAKE---M-APYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHL-LFERDQLDEIKQPVIDAIAEYFGWTEE-

General information: TITO was launched using: RESULT: Template: 2RGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2818 26726 9.48 56.15 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 9.48 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.401

(partial model without unconserved sides chains): PDB file : Tito_2RGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RGH-query.scw PDB file : Tito_Scwrl_2RGH.pdb: