Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MLQNLDSLYQSLSKTEERLEEIIQQ--------EIKLKDYQVEVDSAMSRMNKSYQNLYSERSSSS----REIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSLIDKLTKNSITNQDI---------------YNDILDIRVSGESSIFSDKKLINSIRKLNDAQVL------ELQTLKPNDLVVIRL-------ELNGKKVSLS-NASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVRSIITKKENRQILIVTHNANI---------PVNGDSEWLISMG--DSKEISADQCGSV----DEPNIKPRICTV--MEGGEDAFTNRAKRYGFKQIVE------------- 4B3X Chain:A ((1-363)) MAKVRIYQLAKELGMETQELLELLDQMGVAYKSHASTLEEKDAEAVRELVKEQRGLQEKLAEEERRKSLPRRPPVVVIMG--HVDHGKTTLLDYLRKSRIAEKEGGGITQHVGAFEVKTPQGTVVFIDTPGHEAFTTIRQRGAKVADIAVIVIAADDGIMPQTEEAIAHAKAAGAKLIFAINKIDLPQADPEKVKRQLMERGFVPEEYGGDAIVIPISAKTGQGVQDLLEMILLLAELEDYRADPNAEP-----RGVILESKLDKQAGIIANMLVQEGTFRVGDYVVAGEAYGRIRAMMDADGNQRKEAGPGSAVQVLGFQELPHAGDVVEWVPDLEAAKEIAEERKEERKAREEEEKARRPRTMAELLR

General information: TITO was launched using: RESULT: Template: 4B3X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1236 94310 76.30 324.09 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 76.30 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.301

(partial model without unconserved sides chains): PDB file : Tito_4B3X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4B3X-query.scw PDB file : Tito_Scwrl_4B3X.pdb: