Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MNENPLAVKLPGLELKNPIMPASGCF-GFGKEYGKYYDLNQLGSIMVKATTPNA-RFGNPTPRVAETPS-GMLNAIGLQNPGLDVVMHKLLPELEEYNEL--PIIANVAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVK--HGGIAFGTDSDVAFQLTQAVKKVSSVPVYVKLSPNV-TDIVPIAQAIEAGGADGFTMINTLLG-MRIDLKTRKPIL--ANQTGGLSGPAIKPVAIRLIHQVASI-SSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTD-PYICPKLIKELPVRMSELGIESLERLIKEVREERER 1EP3 Chain:A ((1-311)) MTENNRLSVKLPGLDLKNPIIPASG-CFGFGEEYAKYYDLNKLGSIMVKATTLH-PRFGNPTPRVAETA-SGMLNAIGLQNPGLEVIMTEKLPWLNENFP-ELPIIANVAGSEEADYVAVCAKIGDAANVKAIELNISCPNVKHG--GQAFGTDPEVAAALVKACKAVSKVPLYVKLSPNVT-DIVPIAKAVEAAGADGLTMINTLM-GVRFDLKTRQPILAN--ITGGLSGPAIKPVALKLIHQVAQDVD-IPIIGMGGVANAQDVLEMYMAGASAVAVGTANFA-DPFVCPKIIDKLPELMDQYRIESLESLIQEVKEGKK-

General information: TITO was launched using: RESULT: Template: 1EP3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1799 -6790 -3.77 -22.86 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.90 3D Compatibility (PKB) : -3.77 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_1EP3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EP3-query.scw PDB file : Tito_Scwrl_1EP3.pdb: