Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIVPEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNL-NWTEVQFVKKLIEAGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGAGGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGEEVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAG-EFLRSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA 3VOV Chain:B ((1-295)) --MKVVGLDLGGTKIAAGVFDG-KRLLSKVVVPTPK-EGGERVAEALAEAAERAERE---AGVRGEAIGLGTPGPLDFRRGVIR--PNIPGVQDFPIRRILEEA-TGRPVFLENDANAAALAEHHLGAAQGEESSLYLTVSTGIGGGVVLGGRVLRGERGQGGELGHLTLLPGGPACGCGLEGCLEALAAGRALERDATYAFQRP---------------VDTRELFRLFQAGDPKAERLVLQAARYVGIGLASLVKAFDPGVVVLGGGVALNAPEGYWEALLEAYRRYLQG--WEAPPLRRARLGAEAGLLGAALTAYL--

General information: TITO was launched using: RESULT: Template: 3VOV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1753 -59882 -34.16 -205.78 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -34.16 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_3VOV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VOV-query.scw PDB file : Tito_Scwrl_3VOV.pdb: