Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIKF-NGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSN-LRISDRAHVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERLRLNLDDKNRQFVKMF--DSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVLFEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGPFPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVLSGLETVKICTAYEL-DGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPENARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI 1QF5 Chain:A ((1-431)) GNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTLVIN-GEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSEACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAEKLKEVMEYHNFQLVNYYKAEAV--DYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDFVMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGAGPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLDVLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGLPQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA-

General information: TITO was launched using: RESULT: Template: 1QF5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2529 34584 13.67 81.18 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : 13.67 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_1QF5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QF5-query.scw PDB file : Tito_Scwrl_1QF5.pdb: