Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLLFQSIKSITVPELKEKLL---ENPALLDVRTPAEYRGGHIKGAKNVPLQS-INRY-DG-DKEKTVYVICQSGMRSKQAAKELKKSGYD-VVNVRGGMNQWFDRTVGGK 2KL3 Chain:A ((3-103)) --------PQSDAHVLKSRLEWGEPAFTILDVRDRSTYNDGHIMGAMAMPIEDLVDRASSSLEKSRDIYVYGAGDEQTSQAVNLLRSAGFEHVSELKGGLAAWKAIGGP--

General information: TITO was launched using: RESULT: Template: 2KL3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 448 34104 76.12 362.80 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 76.12 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.571

(partial model without unconserved sides chains): PDB file : Tito_2KL3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2KL3-query.scw PDB file : Tito_Scwrl_2KL3.pdb: