Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFFQGHFPEEPVMPGVLIVEAMAQAGA-VALLSLEQF-----KGKTAYFGGLDKAKFRKKVTPGDTLYLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG 3DOY Chain:B ((14-156)) --IEHILQILPHRYPMLLVDRITELQANQKIVAYKNITFNEDVFNGHFPNKPIFPGVLIVEGMAQSGGFLAFTSLWGFDPEIAKTKIVYFMTIDKVKFRIPVTPGDRLEYHLEVLKHKGMIWQVGGTAQVDGKVVAEAELKAMIA

General information: TITO was launched using: RESULT: Template: 3DOY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 626 -43903 -70.13 -320.46 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -70.13 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_3DOY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DOY-query.scw PDB file : Tito_Scwrl_3DOY.pdb: