Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKDIHPAYQQVLFHDTNADVYFLIGSTIQTKQTKEYQGQVYPYVTLDISSASHPFYTGEVRQASNEGRVASFNKRFARFNRKS------ 1Z4H Chain:A ((1-66)) MQHELQP--------DSLVDLKFIMADT----------GFGKTFIYDRIKSGDLP------KAKVIHGRARWLYRDHCEFKNKLLSRANG

General information: TITO was launched using: RESULT: Template: 1Z4H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 211 -9330 -44.22 -155.49 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -44.22 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.077

(partial model without unconserved sides chains): PDB file : Tito_1Z4H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Z4H-query.scw PDB file : Tito_Scwrl_1Z4H.pdb: