Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNATLNYQPHWVREDFVDFVLQKINATWAWKRVLAEVTAVQSLHTDMVLIKLKPNRNFNFDQVRAGESILLTLLIDG--VYQQRSYSIIEVTTQGEIALGIKVQ-------GLVSRAAQ-LLHVGECVEISQPQGDFTLHQGQQPAILIASGSGVTAIYSLLQQALKQQLEQIHVIYFNRA---EIFHAELKALAEQYPQLQYHFFNTTEQ-KQHLTE-SLLQKLVPDFEQTATYVCGHHGMMQQANEIYTQKGAQ-SQLHQEYFQPLQVTGTHAAQPVIFRRAQQEFLAETNLLGSAEQAGLRPQHGCRMGVCNKCSCTKVSGVTQNLLTGEIEDQPNRPIKLCVSQALSPVTIDL 4EH1 Chain:B ((2-238)) ------------------------------DGRTFVVREKQVESAYVTSFVLVPADGGAVLDYQPGQYIGIEVTPEGSDYREIRQYSLSHASNGREYRISVKREGVGSDNPGLVSHYLHNNVKVGDSVKLYAPAGDFFYVERERPVVLISAGVGATPMQAILHTLAKQNKSGVTYLYACNSAKEHTFAQETAQLIAQQGWMQQ-VWYRDESADDVLQGEMQLAELILPIEDGDFYLCGPIGFMQYVVKQLLALGVDKARIHYEVFGPH-----------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4EH1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1165 -40452 -34.72 -183.04 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -34.72 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_4EH1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EH1-query.scw PDB file : Tito_Scwrl_4EH1.pdb: