Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLVEKLRRKIMQSKTLFLLGLCTIFSTTVSALPLDSKGAKQRLSQAAKQKVFQGKIDLDALVSNDSNDLIVEYNIPSSSVSSGLERRSYIATNKKNLQTRFSRAGGVQVLRDYNNLPLAFYRISNREALIALLNDPNVKAVYPNRINRTTTTESLPLINQPQANTN-GFTGEGSSVAVLDTGVNYLHSDFGCTAVNTPSSTCRVVYSFDSAP--------------------DDGTLDDDGHGSNVSAIVS----------KVATKTKIIGIDVFRKVRSQGKWVSTAYDSDILAGINWAVNNAQT-YNIKAVNLSLGVPGVKYTSECSDSSYGTAFANARA-AGVVPVVASGNDAFP-------------------------------------------------------------------------------------------------------------------------------------DGISSPACVAGAVRVGAVYDSNIGGVSWGNPVKCSDPTTAADKVACFSNGGS-------LVTLLAPGAMITAG-----GYTMGGTSQATPHVAGAIALLRANS----VSPTESIDQTISRLKATGKPIT----DSRTGLVFPRIDLLAATNGLTVN 4I0W Chain:D ((3-466)) --------------------------------------------------------------------------------------------------------------------------------------------------------DSNRASCIPSVWNNYNLTGEGILVGFLDTGIDYTHNAFKDAEG-----NTRIEYIYDLENGVVYDKNKINEALKSEDPFSIVPEIDLSGHGTHVAGIACAGGNINFDNYGVAYKSSIAMVKITGENSL-----RAALSTQLMRGLKFLMDKSNEINKPLVVNISLSTNDGSHNG---SSLLEKYIQTFTQLQKAVIVVAAGNEGNSAHHVGGKMKKEEDLDLNIGDGEKGIILDFFKPVLVDVSVEVISPTGISTGPIELSESYKERFVGREKIVVYSTGPKPFDIQGQTTISILPLGDTITSGGWRIIVRKLNNYEGYFDIWLPGLNERTRFLQPSVYNTLGIPATVEGVISVGSYNFL-------------------NNNLSAFSGRGVVRPEWLIKPDLVAPGENILSTVEEQGFDTKSGTSMAAPQVSGICALLFEWGIIRNNDPFLYGERIKYYLIKGAKRTIFGEAYPNPDLGYGFVCLDRTMELLI--

General information: TITO was launched using: RESULT: Template: 4I0W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1148 11708 10.20 42.57 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain D : 0.59 3D Compatibility (PKB) : 10.20 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_4I0W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4I0W-query.scw PDB file : Tito_Scwrl_4I0W.pdb: