Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKFLNPQYLFQLLSPCLLCEIGTREKYSLCKECWEQLPWLKQTIQRNDQSVLVACHYAYPINRIIQQFKYEQKLHYQTLLAEVLQQLK-FPKVQAIVPMPISKQRLTERGFNQSLLLANLLGKQLKIPVWQPVQRLNEHSQKGL--SRLERFENIEQQFVALTQENRRYRRVLIIDDVITTGSSIHALSQALKQLGCTSIHASCLAAASSTSY 1O57 Chain:B ((102-235)) ----------------------------------------------------------------YVYLTDILGKPSVLSKVGKLFASVFAEREIDVVMTVATK-------GI----PLAYAAASYLNVPVVI-VRKD----GSTVSINYVSGSSNRIQTMSLAKRSMKTGSNVLIIDDFMKAGGTINGMINLLDEFNANVAGIGVLVEA-----

General information: TITO was launched using: RESULT: Template: 1O57.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 641 -38423 -59.94 -304.94 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -59.94 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.432

(partial model without unconserved sides chains): PDB file : Tito_1O57.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O57-query.scw PDB file : Tito_Scwrl_1O57.pdb: