Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEGIKTIGVVGVGVIGASWTALFLYKGFKVKVYGPYPIDEELFKKRIQANLSDLLALDQQTDSSHHLQDIFLNLELYNNLKDAVIDADFIQENAPERLDLKQNLYQEITSYCPEKTLIASSSSGLKVSDFQKDATHPERIFLGHPFNPPHLLPLVEIVGGKLTDPQILKKASEFYQSLGKHPIVLNKEVKGHVANRLQAALWREAFSLVKEGVCSAEDVDIAITSGPGLRWALFGPYINMELANQKGFKEAIHHLGPPMTEWWNDMQNFQHSEETTELLEEQTKELLTHYKDIDLSQKRDKGLVDILKLKQQLELD 4PZC Chain:C ((2-249)) --SIRTVGIVGAGTMGNGIAQACAVVGLNVVMVDISDAAVQKGVATVASSLDRLIKKEKLTEA--DKASALARIKGSTSY-DDLKATDIVIEAATENYDLKVKILKQIDGIVGENVIIASNTSSISITKLAAVTSRADRFIGMHFFNPVPVMALVELIRGLQTSDTTHAAVEALSKQLGKYPITV-KNSPGFVVNRILCPMINEAFCVLGEGLASPEEIDEGMKLGCNHP---IGPLALADMIGLDTMLAVMEVLYT-----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4PZC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1272 -138829 -109.14 -559.79 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.72 3D Compatibility (PKB) : -109.14 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_4PZC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PZC-query.scw PDB file : Tito_Scwrl_4PZC.pdb: