Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WFFGAIRRSDAEKQLLYSENKTGSFLIRESESQKGEFSLSVLD-----GAVVKHYRIKRLDEGGFFLTRRRIFSTLNEFVSHY 1CWD Chain:L ((3-85)) WFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHY

General information: TITO was launched using: RESULT: Template: 1CWD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 246 -7359 -29.91 -94.34 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain L : 0.87 3D Compatibility (PKB) : -29.91 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.613

(partial model without unconserved sides chains): PDB file : Tito_1CWD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CWD-query.scw PDB file : Tito_Scwrl_1CWD.pdb: