Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence FFFGNITREEAEDYLVQGGMSDGLYLLRQSRNYLGGFALSVAHGRKAHHYTIERELNGTYAIAGGRTHASPADLCHYH 3N84 Chain:B ((9-84)) WFFGKIPRAKAEEMLSKQ-RHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFN-SLNELVDYH

General information: TITO was launched using: RESULT: Template: 3N84.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 269 -41076 -152.70 -540.47 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -152.70 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_3N84.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N84-query.scw PDB file : Tito_Scwrl_3N84.pdb: