Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHY 1SKJ Chain:A ((6-88)) WYFGKITRRESERLLLNPENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFSSLQQLVAYY

General information: TITO was launched using: RESULT: Template: 1SKJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 295 -38538 -130.64 -464.31 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.92 3D Compatibility (PKB) : -130.64 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_1SKJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SKJ-query.scw PDB file : Tito_Scwrl_1SKJ.pdb: