Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINSYYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR-FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLK-DGALDMEGLRAKLTDKVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFAFSAHK-MAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPNMAGAIGLATAVDYLEKIGMDAIEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVIAFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDKLVDALQKTKEFFNGTF 5B7U Chain:A ((23-417)) --IPEDVRKDIPLT------NEVIYFDNTATSLTPKPVVEAMDEYYLKYRANVHRGVHRLSQMATHKYEESRKIVADFIGAK-FEEIVFTKNTSESLNLVALGLGHI-FKRGDKIVTTPYEHHSDLLPWQRLATKLGLKLEFIEGDDEGNLDLSDAEKKI-KGAKLVAVQHVSNALGVIHEVEELGKIAKDEGAIFVVDAAQSAGHMEVNVKKLHADFLAFSGHKGPMGPTGIGVLYIREEFFDTFEPPLIGGGTIEDVSLDGYKLTEPPERFEAGTPNIGGAIGLAAGIRYIERIGLGRIERQEHKLVKRTTEGLDEL-EVPWYGPRNLKKHAGVVSFNVPGLHPHDVAAILDDHSIMVRSGHH-ALPVMKKLGINGTVRASFHVYNSLEEVETFLGVMEELVKGL----

General information: TITO was launched using: RESULT: Template: 5B7U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2349 -46513 -19.80 -118.96 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -19.80 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_5B7U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5B7U-query.scw PDB file : Tito_Scwrl_5B7U.pdb: